Partial network representation of gene sets listed in the KEGG pathway database from GSEA C2 gene set annotations. Our investigation illustrates the process of generating new hypotheses and informing future experimental research by repurposing multiple space-flown datasets. Although we did not perform any functional studies, our findings revealed a mild hypoxic phenotype in AEM, possibly due to atmospheric carbon dioxide that was increased to match conditions in spaceflight.
Using a systems biology approach, we observed that AEM housing was associated with significant transcriptomic differences, including reduced metabolism, altered immune responses, and activation of possible tumorigenic pathways. To investigate the innate global transcriptomic patterns of rodents housed in spaceflight-matched AEM compared to standard vivarium cages we utilized publicly available data from the NASA GeneLab repository. There are limited studies examining the biological response invariably due to the configuration of AEM and vivarium housing. Ground control AEMs are engineered to match the spaceflight environment. Rodent spaceflight experiments involve both standard vivarium cages and animal enclosure modules (AEMs), which are cages used to house rodents in spaceflight. In order to understand the impact of each environmental component on astronauts it is important to investigate potential influences in isolation. Spaceflight introduces a combination of environmental stressors, including microgravity, ionizing radiation, changes in diet and altered atmospheric gas composition.
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